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KMID : 0613820020120040423
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Journal of Life Science
2002 Volume.12 No. 4 p.423 ~ p.426
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Enantioselective Resolution for the Preparation of Chiral para-Nitrostyrene Oxide by Microbial Epoxide Hydrolase in an Organic Solvent
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Bae Hyun-Chul
Kim Hyun-Sook
Lee Soo-Jung
Lee Eun-Yeol
Yang Seung-Taek
Kim Hee-Sook
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Abstract
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Enantioselective resolution of racemic para-nitrostyrene oxide was investigated using epoxide hydrolase activity of Aspergillus niger LK for the production of optically pure (S)-para-nitrostyrene oxide. To overcome the poor solubility of the substrate, enantioselective hydrolysis in an organic solvent was attempted under optimized reaction conditions including reaction temperature and water content. (S)-para-Nitrostyrene oxide with high optical purity (> 99% ee) was obtained at 37 % yield using fungal epoxide hydrolase-catalyzed enantioselective resolution
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KEYWORD
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epoxide hydrolase, para-nitrostyrene oxide, enantioselective resolution, organic solvent, Aspergillus niger
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